The incubation time in liquid paraffin ought to be extended more than enough to get enough infiltration, although not any more than necessary to be able to stop prolonged exposure of your tissue to heat. For a similar rationale, it is suggested which the temperature in the liquid paraffin need to be no more than two–four C previously mentioned th
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He at times mounted an embryo in copyright balsam and closed it with glass addresses in a chamber slide so that he could analyze it from either side (dehydration and clearing had been already proven procedures [43, p. twelve]).This section collects any details citations, knowledge availability statements, or supplementary resources integrated in
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